TransPure™ Proteinase K

Salient Features

• Ultra-High Purity
  Recombinant origin, ≥ 99% purity by SDS-PAGE; completely free of DNase and RNase contamination to protect nucleic acids during downstream applications.
• Robust Activity Profile
  Active over pH 6.5–9.5 and temperatures from 37 °C up to 65 °C; retains >90% activity after 1 hour at 55 °C.
• Wide Substrate Specificity
  Efficiently digests native and denatured proteins, including challenging histone and membrane-bound proteins.
• Enhanced Stability
  Lyophilized format for long-term storage; formulated with stabilizers to maintain full activity through multiple freeze–thaw cycles.
• Rapid Digestion Kinetics
  Completes protein digestion in 10–30 minutes (depending on substrate and conditions), accelerating overall workflow throughput.
• DNase/RNase Free
  Guaranteed nuclease-free to preserve integrity of genomic and transcriptomic samples.

Available Sizes

1. 22 mg lyophilized powder (Cat No- TPRK 22)
2. 33 mg lyophilized powder (Cat No- TPRK 33)
3. 100 mg lyophilized powder (Cat No- TPRK 100)
4. 500 mg lyophilized powder (Cat No- TPRK 500)
5. 1000 mg lyophilized powder (Cat No- TPRK 1000)

Storage & Stability

• Storage: –20 °C, protected from moisture.
• Shelf Life: ≥ 24 months from date of manufacture in unopened vial.
• Working Solution: Reconstitute in sterile water or buffer; store aliquots at –20 °C for up to 6 months, or 4 °C for up to 1 week.

Applications

• Genomic DNA & RNA Isolation: Complete removal of contaminating proteins and nucleases for high-yield, high-purity nucleic acid preps.
• Histology & Tissue Digestion: Rapidly dissociate tissue samples for single-cell suspensions or extracellular matrix studies.
• Proteomics Sample Prep: Efficient proteolysis for LC-MS/MS analysis; ideal for in-solution and in-gel digestions.
• Cell Lysis & Protein Extraction: Gentle yet thorough digestion of cellular proteins for Western blotting or immunoprecipitation workflows.
• Antibody Fragment Generation: Cleavage of IgG to generate Fab and F(ab′)₂ fragments under controlled conditions.